9AZV

CH67 Fab bound to A/Massachusetts/1/1990 influenza hemagglutinin head with a G189E mutation (2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 2
Unit cell lengths	76.277, 93.467, 108.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 3.160
R-factor	0.2421
Rwork	0.237
R-free	0.28730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.484
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.030	3.350
High resolution limit [Å]	3.160	3.160
Number of reflections	13883	2181
<I/σ(I)>	10.81	2.18
Completeness [%]	99.7
Redundancy	5.8
CC(1/2)	0.996	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG 8K, 100 mM HEPES, pH 7, 100 mM MgSO4