9AZR

Lineage 860 UCA Fab bound to A/Massachusetts/1/1990 hemagglutinin head

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-20
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	202.543, 71.863, 65.402
Unit cell angles	90.00, 104.87, 90.00

Refinement procedure

Resolution	48.940 - 2.300
R-factor	0.2306
Rwork	0.229
R-free	0.27030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.546
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.940	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	39860	5923
<I/σ(I)>	10.35
Completeness [%]	98.0
Redundancy	6.7
CC(1/2)	0.995	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	20% PEG 4k, 200 mM imidazole, pH 7.4, HCl