9AZ2
Crystal structure of PF3257 peroxidase from Pseudomonas fluorescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2019-07-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 78.334, 114.747, 143.717 |
| Unit cell angles | 83.31, 77.44, 86.26 |
Refinement procedure
| Resolution | 19.990 - 2.520 |
| R-factor | 0.1982 |
| Rwork | 0.198 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.544 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.560 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.185 | 0.759 |
| Rpim | 0.130 | 0.525 |
| Number of reflections | 158500 | 7703 |
| <I/σ(I)> | 5.5 | 1.25 |
| Completeness [%] | 96.8 | 94.9 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.525 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 15 mg/mL protein, 0.1 M Bis-Tris pH 5.5, 0.2 M magnesium chloride, 25% w/v PEG 3350, cryoprotectant: paratone |
