Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.170, 51.790, 59.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.040 - 1.900 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.040 | 35.040 | 1.950 |
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.079 | 0.054 | 1.694 |
| Rmeas | 0.083 | 0.059 | 1.767 |
| Number of reflections | 11085 | 149 | 802 |
| <I/σ(I)> | 14.78 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 11.7 | ||
| CC(1/2) | 0.997 | 0.997 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.001 M Zinc Sulfate, 0.05M HEPES (pH 7.8), 28% PEG 600 |
