9AXQ
Crystal Structure of HY11-7E1_Hu3 Fab in the Apo Conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-27 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.581, 151.103, 104.604 |
Unit cell angles | 90.00, 91.14, 90.00 |
Refinement procedure
Resolution | 42.830 - 2.200 |
R-factor | 0.2091 |
Rwork | 0.208 |
R-free | 0.23760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.583 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.132 | 0.101 | 0.876 |
Rmeas | 0.161 | 0.122 | 1.134 |
Rpim | 0.091 | 0.068 | 0.711 |
Total number of observations | 284536 | ||
Number of reflections | 111677 | 5856 | 4405 |
<I/σ(I)> | 7.4 | ||
Completeness [%] | 95.7 | 98.3 | 76.1 |
Redundancy | 2.5 | 3 | 1.9 |
CC(1/2) | 0.978 | 0.980 | 0.417 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M ammonium sulfate, 0.1M sodium acetate pH 4.6, 25% PEG 4K |