Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.374, 299.672, 144.659 |
| Unit cell angles | 90.00, 112.89, 90.00 |
Refinement procedure
| Resolution | 48.900 - 3.440 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.534 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | DIMPLE |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.560 | 3.500 |
| High resolution limit [Å] | 3.430 | 3.440 |
| Rmerge | 0.313 | 1.269 |
| Rmeas | 0.339 | 1.374 |
| Rpim | 0.131 | 0.524 |
| Total number of observations | 45997 | |
| Number of reflections | 139213 | 6822 |
| <I/σ(I)> | 6.4 | 1.6 |
| Completeness [%] | 99.3 | |
| Redundancy | 6.6 | 6.7 |
| CC(1/2) | 0.972 | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM |
