9AW3
Yeast 20S proteasome soaked with MA9 crude extract
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 131.964, 301.458, 142.649 |
| Unit cell angles | 90.00, 111.98, 90.00 |
Refinement procedure
| Resolution | 29.840 - 3.420 |
| R-factor | 0.2171 |
| Rwork | 0.215 |
| R-free | 0.25940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.476 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21-5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.840 | 3.480 |
| High resolution limit [Å] | 3.420 | 3.420 |
| Rmerge | 0.157 | 0.800 |
| Rmeas | 0.190 | 0.988 |
| Rpim | 0.106 | 0.573 |
| Number of reflections | 136674 | 6705 |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 98.4 | 97.3 |
| Redundancy | 3.1 | 2.8 |
| CC(1/2) | 0.990 | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MES 100 mM pH 5.5-6.9, MPD 8-18% and magnesium acetate 20 mM |
