9AVO
The crystal structure of an engineered Protein GD with Human Kappa Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 134.971, 134.971, 339.568 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 86.730 - 3.000 |
| R-factor | 0.2158 |
| Rwork | 0.214 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.730 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.167 | |
| Rpim | 0.049 | |
| Number of reflections | 31838 | 4540 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.5 | 13 |
| CC(1/2) | 0.998 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.1M Sodium cacodylate pH 6.8 and 0.8M Sodium citrate tribasic |
