9AVM

Crystal Structure of CARD9 coiled-coil K156-K214

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	27.330, 48.830, 55.630
Unit cell angles	71.41, 79.27, 77.05

Refinement procedure

Resolution	26.430 - 1.790
R-factor	0.2443
Rwork	0.242
R-free	0.29230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.820
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (20-MAY-2020))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.430	26.400	1.900
High resolution limit [Å]	1.790	5.270	1.790
Rmerge	0.044	0.030	0.520
Rmeas	0.060	0.040	0.712
Number of reflections	23908	943	3878
<I/σ(I)>	9.72
Completeness [%]	96.0
Redundancy	2.03
CC(1/2)	0.997	0.997	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	8-14% PEG 3350