9AVH
Crystal structure of an aryl-alcohol-oxidase from Bjerkandera adusta.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.035, 82.746, 116.837 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.526 - 1.800 |
| Rwork | 0.143 |
| R-free | 0.18830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.665 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.840 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.097 | 0.843 |
| Number of reflections | 50514 | 7207 |
| <I/σ(I)> | 12.2 | 2.7 |
| Completeness [%] | 98.9 | 98.2 |
| Redundancy | 6.7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 12% v/v glycerol, 1.5 M ammonium sulfate, 0.1 mM Tris/HCl, pH 8.5 |
