9AVD

Mitochondrial fission 1 protein Fis1 structure T34D mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-05-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 41 21 2
Unit cell lengths	43.510, 43.510, 120.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.300 - 2.510
R-factor	0.2373
Rwork	0.234
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.091
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.300	35.300	2.580
High resolution limit [Å]	2.510	11.230	2.510
Rmerge	0.094	0.053	1.816
Rmeas	0.098	0.056	1.873
Number of reflections	4385	66	327
<I/σ(I)>	14.29
Completeness [%]	100.0
Redundancy	16.2
CC(1/2)	0.998	0.998	0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2M Sodium Bromide, 20% PEG 3350