9AUS
Crystal structure of loop-closed dumbbell RNA bridged by glycine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-22 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 1 |
Unit cell lengths | 38.514, 82.085, 92.668 |
Unit cell angles | 63.95, 88.69, 88.40 |
Refinement procedure
Resolution | 46.070 - 2.070 |
R-factor | 0.2222 |
Rwork | 0.221 |
R-free | 0.25380 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.576 |
Data reduction software | MOSFLM (1.20.1_4487) |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.070 | 2.144 |
High resolution limit [Å] | 2.070 | 2.070 |
Number of reflections | 57272 | 5776 |
<I/σ(I)> | 6.55 | |
Completeness [%] | 91.1 | |
Redundancy | 3.9 | |
CC(1/2) | 0.992 | 0.591 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 277.15 | 0.2 M Lithium sulfate monohydrate, 20% w/v Polyethylene glycol 3,350, |