9AUA
PLP-dependent BesB holoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 58.640, 58.640, 249.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.076 - 1.290 |
| R-factor | 0.1681 |
| Rwork | 0.167 |
| R-free | 0.18370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.838 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.780 | 1.310 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rpim | 0.037 | 0.712 |
| Number of reflections | 126887 | 6174 |
| <I/σ(I)> | 12.1 | 1.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 17 | 17.1 |
| CC(1/2) | 0.999 | 0.375 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.16 M magnesium acetate, 0.08 M Tris, 14-22% PEG 8000, and 15-20% glycerol, 4-10 mg/mL SAcBesB |
