9ATC
ATCASE Y165F MUTANT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 300 |
Detector technology | AREA DETECTOR |
Collection date | 1994-06 |
Detector | SIEMENS |
Spacegroup name | R 3 2 |
Unit cell lengths | 100.600, 100.600, 100.600 |
Unit cell angles | 81.40, 81.40, 81.40 |
Refinement procedure
Resolution | 10.000 - 2.400 |
R-factor | 0.252 |
Rwork | 0.252 |
R-free | 0.34400 |
Structure solution method | MR |
Starting model (for MR) | ATCASE WILDTYPE T (1RAI) |
RMSD bond length | 0.013 |
RMSD bond angle | 25.313 * |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.480 |
High resolution limit [Å] | 2.400 | 2.340 |
Rmerge | 0.074 * | 0.133 * |
Total number of observations | 160964 * | |
Number of reflections | 21881 | 1980 * |
<I/σ(I)> | 14.3 | 1.6 |
Completeness [%] | 83.0 | 42 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.45 * | 18 * | pH 6.3 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | HEPES | 0.01 (M) | |
3 | 1 | reservoir | Tris | 50 (mM) | |
4 | 1 | reservoir | imidazole | 50 (mM) |