9ARO

Crystal structure of AF9 YEATS domain in complex with acetylated at K1007 MOZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2023-06-09
Detector	RDI CMOS_8M
Wavelength(s)	1.0722
Spacegroup name	P 1
Unit cell lengths	44.740, 62.600, 75.658
Unit cell angles	90.11, 107.32, 90.00

Refinement procedure

Resolution	47.400 - 2.300
R-factor	0.2507
Rwork	0.250
R-free	0.27262
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.713
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.400	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.069	0.517
Rmeas	0.097	0.730
Rpim	0.068	0.515
Total number of observations		6239
Number of reflections	31141	3147
<I/σ(I)>	7.2	1.5
Completeness [%]	89.2
Redundancy	2	2
CC(1/2)	0.975	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	0.2 M Sodium formate pH 7.2, 20% (w/v) PEG 3350