9ANT
ANTENNAPEDIA HOMEODOMAIN-DNA COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-15 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 61.050, 77.750, 94.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.23900 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 18.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.088 * |
| Total number of observations | 309545 * |
| Number of reflections | 17753 |
| Completeness [%] | 97.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 1-5% MPD 20 MM BIS-TRIS-PROPANE, PH 7.0 10 MM NICL2 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | NICL2 | ||
| 2 | 1 | 1 | BIS-TRIS-PROPANE | ||
| 3 | 1 | 1 | MPD | ||
| 4 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | DNA | 5.7 (mM) | |
| 3 | 1 | reservoir | 1-5 (%) | ||
| 4 | 1 | reservoir | Bis-Tris propane | 20 (mM) | pH7.0 |
| 5 | 1 | reservoir | 10 (mM) |
