9ZLG

Crystal structure of DCAF1 in complex with SDIPTAC C9

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002+
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-09-05
Detector	DECTRIS EIGER R 1M
Wavelength(s)	1.54184
Spacegroup name	C 1 2 1
Unit cell lengths	120.801, 120.867, 208.988
Unit cell angles	90.00, 89.99, 90.00

Refinement procedure

Resolution	30.180 - 2.300
R-factor	0.2434
Rwork	0.243
R-free	0.28910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.828
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.180	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.169	0.987
Rmeas	0.194	1.142
Rpim	0.092	0.557
Number of reflections	118231	17966
<I/σ(I)>	5.8
Completeness [%]	89.1
Redundancy	4
CC(1/2)	0.983	0.648

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	294.15	5% w/v 1-ethyl-3-methylimidazolium acetate, 15% w/v PEG 20,000, pH 6.5