9ZLE

Crystal structure of DCAF1 in complex with SDIPTAC C8

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002+
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-31
Detector	DECTRIS EIGER R 1M
Wavelength(s)	1.54184
Spacegroup name	C 1 2 1
Unit cell lengths	146.592, 84.785, 221.466
Unit cell angles	90.00, 89.98, 90.00

Refinement procedure

Resolution	29.050 - 2.550
R-factor	0.2618
Rwork	0.261
R-free	0.30540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.470
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.060	2.690
High resolution limit [Å]	2.550	2.550
Rmerge	0.281	1.551
Rmeas	0.298	1.636
Rpim	0.097	0.519
Number of reflections	88221	12697
<I/σ(I)>	6.2
Completeness [%]	99.3
Redundancy	9.2
CC(1/2)	0.987	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	294.15	1.0 M ammonium tartrate dibasic, 0.1 M sodium acetate trihydrate, pH 4.6