9ZCS

Crystal structure of HOPS subunits Vps33 and Vps16 in complex with the Nyv1 and Vam3 SNARE motifs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	100.844, 100.844, 312.412
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.600 - 3.190
R-factor	0.1798
Rwork	0.177
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.971
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.600	3.380
High resolution limit [Å]	3.190	3.190
Rmerge	0.304	1.943
Rmeas	0.312	1.994
Rpim	0.069	0.442
Total number of observations	557359	87050
Number of reflections	27836	4382
<I/σ(I)>	11.2	1.9
Completeness [%]	99.8
Redundancy	20	19.9
CC(1/2)	0.998	0.779

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.8M K Tartrate, 0.1M Tris-HCl pH8.5, 0.5% w/v PEG 5000 MME