9Z9F
Structure of FabS1CE2_ER-2a in complex with the extracellular region of EGFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-07-18 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.919716 |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.533, 113.846, 118.767 |
| Unit cell angles | 114.16, 95.24, 104.88 |
Refinement procedure
| Resolution | 34.420 - 3.060 |
| R-factor | 0.2144 |
| Rwork | 0.213 |
| R-free | 0.24870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.677 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.660 | 3.250 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.241 | 1.468 |
| Rmeas | 0.284 | 1.721 |
| Rpim | 0.148 | 0.891 |
| Number of reflections | 55114 | 8023 |
| <I/σ(I)> | 4.4 | 0.9 |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.975 | 0.409 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M ammonium citrate, 0.1 M bis-tris propane pH 6.5, cryoprotected with this buffer plus 30% ethylene glycol |
