9Z70

Crystal structure of shorter construct of SHP2 unbound N-SH2 domain (Y66 in blocking conformation)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-10-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00004
Spacegroup name	P 43 21 2
Unit cell lengths	57.826, 57.826, 81.371
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.140 - 1.730
R-factor	0.1982
Rwork	0.195
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.262
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.140	1.790
High resolution limit [Å]	1.730	1.730
Number of reflections	15009	1333
<I/σ(I)>	9.4
Completeness [%]	99.9	99.55
Redundancy	25.2
CC(1/2)	0.997	0.184

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.6 M Sodium citrate tribasic dihydrate pH 6.5