9YMG
Human KIF18A-DARPin fusion protein bound to AMP-PNP and tubulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 105.380, 135.470, 117.670 |
| Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
| Resolution | 48.673 - 2.406 |
| Rwork | 0.191 |
| R-free | 0.23770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.061 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.673 | 2.450 |
| High resolution limit [Å] | 2.406 | 2.410 |
| Rmerge | 0.091 | |
| Number of reflections | 121098 | 5981 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.999 | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.2 M ammonium nitrate, 0.1 M sodium acetate pH 5.6, 20% (w/v) PEG Smear Low (5% PEG 400, 5% PEG 500 MME, 5% PEG 600, 5% PEG 1000), 5% (v/v) ethylene glycol |
