9YLP

BCL11A ZF2-3 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGTCCA (C2221 Crystal Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91990
Spacegroup name	C 2 2 21
Unit cell lengths	36.899, 69.554, 129.174
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.780 - 2.040
R-factor	0.2113
Rwork	0.209
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.638
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.780	2.080
High resolution limit [Å]	2.040	2.040
Rmerge	0.093
Rpim	0.027	0.522
Number of reflections	20363	1070
<I/σ(I)>	11.9	1.5
Completeness [%]	100.0	100
Redundancy	13.3	12.9
CC(1/2)	0.999	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	278	28 % w/v PEG 4000, 0.1 M Sodium citrate (pH 6.5), 0.1 M Magnesium acetate tetrahydrate, 0.1 M Ammonium sulfate