9YLM

BCL11A ZF4-6 in Complex with a DNA Sequence Observed in the Human Globin Locus Containing Motif TGTCCA (12-mer)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92017
Spacegroup name	P 41 21 2
Unit cell lengths	56.617, 56.617, 154.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.950 - 1.910
R-factor	0.2068
Rwork	0.205
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.553
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.950	2.150
High resolution limit [Å]	1.910	1.910
Rmerge	0.093
Rpim	0.028	0.622
Number of reflections	14526	726
<I/σ(I)>	14.1	1.4
Completeness [%]	71.0
Redundancy	13
CC(1/2)	0.988	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	292	28 % w/v PEG 4000, 0.1 M Sodium citrate (pH 5.2), 0.2 M Ammonium acetate