9YK5
100K X-ray structure of mixed metal D132N Bacillus halodurans RNase H1 complex with RNA/DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-06-11 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.619, 37.217, 62.310 |
| Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
| Resolution | 26.200 - 2.000 |
| R-factor | 0.255 |
| Rwork | 0.254 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.820 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.900 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.137 | 0.686 |
| Rpim | 0.082 | 0.373 |
| Number of reflections | 12959 | 1294 |
| <I/σ(I)> | 11.5 | 1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 4.3 | 4.4 |
| CC(1/2) | 0.867 | 0.624 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000 |
