9YK1
Room-temperature X-ray structure of D132N Bacillus halodurans RNase H1 in complex with RNA/DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2024-07-08 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.184, 37.743, 62.629 |
| Unit cell angles | 90.00, 96.33, 90.00 |
Refinement procedure
| Resolution | 23.530 - 1.800 |
| R-factor | 0.1866 |
| Rwork | 0.185 |
| R-free | 0.21630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.533 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | 0.678 |
| Rpim | 0.052 | 0.385 |
| Number of reflections | 17367 | 1693 |
| <I/σ(I)> | 13.6 | 1.04 |
| Completeness [%] | 96.8 | 94.1 |
| Redundancy | 4.1 | 3.9 |
| CC(1/2) | 0.949 | 0.638 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.2 M NaCl, and 12-16% PEG 10,000 |
