9YCW

Crystal structure of USP16 ZnF-UBP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 31
Unit cell lengths	84.390, 84.390, 50.432
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.510 - 1.800
R-factor	0.1377
Rwork	0.135
R-free	0.17200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.510	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.055	0.661
Rmeas		0.897
Rpim		0.603
Number of reflections	37022	7276
<I/σ(I)>	11.5	1.7
Completeness [%]	99.5
Redundancy	5
CC(1/2)	0.998	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% (w/v) Glycerol