9YC2

Crystal structure of USP49 ZnF-UBP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	35.739, 47.220, 55.247
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.900 - 1.400
R-factor	0.1551
Rwork	0.153
R-free	0.19170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.308
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.220	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.065	0.923
Rmeas	0.081	1.158
Rpim	0.048	0.693
Number of reflections	18997	4689
<I/σ(I)>	11.2	1.7
Completeness [%]	100.0
Redundancy	5
CC(1/2)	0.999	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	281	0.2 M sodium chloride; 2 M ammonium sulphate; 0.1 M sodium cacodylate