9Y1X
Crystal structure of NRas-G12D in complex with GDP and IACS-56676
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-02-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.813, 46.432, 80.082 |
| Unit cell angles | 90.00, 88.93, 90.00 |
Refinement procedure
| Resolution | 42.810 - 1.720 |
| R-factor | 0.1966 |
| Rwork | 0.194 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.137 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.810 | 1.770 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Number of reflections | 33620 | 152 |
| <I/σ(I)> | 2.46 | |
| Completeness [%] | 100.0 | 99.65 |
| Redundancy | 4.5 | |
| CC(1/2) | 0.976 | 0.695 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.1M Sodium Citrate pH5.5, 0.1M MgCl2, 16-22% Peg 4000 |
