9Y0W

Crystal Structure of human MAIT A-F7 TCR-MR1*05-5-OP-RU complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	213.432, 69.501, 143.205
Unit cell angles	90.00, 103.94, 90.00

Refinement procedure

Resolution	48.980 - 2.400
R-factor	0.1803
Rwork	0.179
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.521
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.980	2.450
High resolution limit [Å]	2.400	2.400
Number of reflections	79884	4517
<I/σ(I)>	12.2
Completeness [%]	99.8
Redundancy	3.5
CC(1/2)	0.998	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, BTP, CaCl2