9XZI
Crystal Structure of the SUZ12-RBBP4-PHF19-EPOP PRC2.1 Subcomplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 148.838, 148.838, 290.672 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.450 - 2.691 |
| R-factor | 0.2108 |
| Rwork | 0.209 |
| R-free | 0.25280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.990 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.4 (20-APR-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 2.750 |
| High resolution limit [Å] | 2.691 | 2.691 |
| Rmeas | 0.142 | |
| Rpim | 0.032 | |
| Number of reflections | 34574 | 34574 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 99.8 | |
| Redundancy | 19.1 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Sodium Citrate, Sodium Acetate, PEG 4000 |
