9XMY
Crystal structure of ASCT D62N mutant from Trypanosoma brucei in complex with succinyl-CoA and acetylacetone.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.900000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.222, 164.833, 189.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.21182 |
| Rwork | 0.207 |
| R-free | 0.29575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.630 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 59146 | 9350 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 99.1 | 99.1 |
| Redundancy | 6.6 | |
| CC(1/2) | 0.997 | 0.897 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 0.05M HEPES-NAOH BUFFER, 18% (W/V) PEG 3350, 0.35M CACL2 |
