9XEG
Structure of the Mid-Cap-627 linker domains of the H5N1 influenza A PB2 subunit in complex with onradivir
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.559, 59.377, 53.953 |
| Unit cell angles | 90.00, 107.57, 90.00 |
Refinement procedure
| Resolution | 51.440 - 1.450 |
| R-factor | 0.1237 |
| Rwork | 0.121 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.380 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.111 | |
| Number of reflections | 42193 | 1609 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 97.4 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M HEPES, pH 7.0, 30% v/v Jeffamine ED-2001, pH 7.0 |
