9WIW

Crystal structure of a Escherichia phage UPEC07 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-07-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979183
Spacegroup name	P 43 21 2
Unit cell lengths	64.471, 64.471, 54.539
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.640 - 2.560
R-factor	0.234
Rwork	0.230
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.299
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.640	2.700
High resolution limit [Å]	2.560	2.560
Number of reflections	4014	565
<I/σ(I)>	29.1	4
Completeness [%]	100.0
Redundancy	44.1
CC(1/2)	1.000	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M Ammonium sulfate 1.0 M Lithium sulfate 0.1 M Sodium citrate