9WGU

Crystal structure of Cbl-b bound to compound

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	56.655, 102.781, 74.647
Unit cell angles	90.00, 90.25, 90.00

Refinement procedure

Resolution	49.665 - 1.950
Rwork	0.174
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.746
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.665	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.075
Number of reflections	61298	4315
<I/σ(I)>	10.3
Completeness [%]	98.5
Redundancy	3.4
CC(1/2)	0.996	0.656

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES pH7.5, 8% EG, 10% PEG8,000