9VZY
Crystal structure of Mycobacterium tuberculosis Rv2514c-Rv2515c toxin antitoxin system.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-12-06 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 122.087, 202.329, 222.999 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 3.200 |
| R-factor | 0.2563 |
| Rwork | 0.255 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.522 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.176 | 1.399 |
| Rmeas | 0.192 | 1.520 |
| Rpim | 0.075 | 0.588 |
| Total number of observations | 298387 | 43801 |
| Number of reflections | 45853 | 6651 |
| <I/σ(I)> | 6.9 | 1.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 | 6.6 |
| CC(1/2) | 0.995 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.8 M Potassium sodium tartrate tetrahydrate, 0.1 M Sodium HEPES pH 7.5 |
