9VRQ

Crystal structure of FOXC2/NFAT1 complex bound to ARRE2 DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	CCD
Collection date	2020-10-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	65.980, 77.343, 117.058
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.670 - 2.800
R-factor	0.2393
Rwork	0.237
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.668
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.670	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.230	0.720
Number of reflections	15281	1483
<I/σ(I)>	12.97	3.44
Completeness [%]	99.9
Redundancy	12.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	50 mM MES 5.93, 200 mM NaCl, 10 mM MgCl2, 1 mM TCEP and 12-15% PEG 4K (w/v)