9VPQ
Crystal structure of METTL9 in complex with compound 15k
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.394, 45.651, 106.895 |
| Unit cell angles | 90.00, 123.23, 90.00 |
Refinement procedure
| Resolution | 35.570 - 2.280 |
| R-factor | 0.2235 |
| Rwork | 0.219 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.461 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.7) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.500 | 2.400 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 26532 | 3858 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.2 | |
| CC(1/2) | 0.998 | 0.895 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 0.1 M BICINE pH 9.0, 2% 1,4-Dioxane, 12% w/v PEG 20000, 12% Glycerol, 0.01 M TCEP |
