9VP0

Structural insights into IL-31 signaling inhibition by a neutralization antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-23
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	42.190, 100.814, 128.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.460 - 2.100
R-factor	0.1929
Rwork	0.191
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.927
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Number of reflections	31471	9970
<I/σ(I)>	13.9	3.7
Completeness [%]	95.4	99.6
Redundancy	6.6
CC(1/2)	0.997	0.925

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M sodium iodide, 0.1 M Bis-Tris propane, 25% PEG3350, pH 8.5