9VNP

The complexes of IFN-gamma and A01BM-03 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-14
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	127.328, 59.103, 77.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 3.020
Rwork	0.229
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	10.000	2.870
High resolution limit [Å]	2.800	6.900	2.800
Rmerge	0.279	0.120	1.037
Rmeas	0.303	0.132	1.208
Rpim	0.117	0.053	0.606
Total number of observations	97305
Number of reflections	15201	1126	850
<I/σ(I)>	4.1
Completeness [%]	98.8	98.5	84.3
Redundancy	6.4	5.9	3.2
CC(1/2)	0.983	0.989	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M BIS-TRIS pH 7.0 and 22% (w/v) Polyethylene glycol 1500