9VLM
The X-RAY co-crystal structure of human FGFR2 and covalent inhibitor 10a
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2024-04-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.982, 47.563, 108.904 |
| Unit cell angles | 90.00, 101.57, 90.00 |
Refinement procedure
| Resolution | 53.340 - 2.260 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.765 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.690 | 2.343 |
| High resolution limit [Å] | 2.260 | 2.263 |
| Rmerge | 0.058 | 0.624 |
| Number of reflections | 28979 | 2845 |
| <I/σ(I)> | 14.63 | 2.29 |
| Completeness [%] | 96.5 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.998 | 0.769 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22% (w/v) PEG 8000, and 0.1 M Tris-HCl, pH 8.0 |
