9VKI
Oligosaccharide sulfotransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.057, 66.899, 102.284 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.990 - 3.000 |
| R-factor | 0.2178 |
| Rwork | 0.215 |
| R-free | 0.27150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.459 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.990 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.187 | 0.568 |
| Rpim | 0.161 | |
| Number of reflections | 9012 | 1422 |
| <I/σ(I)> | 2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.7 | |
| CC(1/2) | 0.994 | 0.921 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 289.15 | 20% w/v PEG2000MME 100mM Tris/200mM TMANO pH=8.5 |
