9VJK
Crystal Structure of an Antigen-Binding Fragment of Monoclonal Antibody 10E6 against Sulfonamides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2019-11-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.930, 59.450, 62.610 |
| Unit cell angles | 90.00, 100.48, 90.00 |
Refinement procedure
| Resolution | 30.780 - 2.510 |
| R-factor | 0.2127 |
| Rwork | 0.211 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.553 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 2.610 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Number of reflections | 14243 | 970 |
| <I/σ(I)> | 6.25 | |
| Completeness [%] | 98.2 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.866 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 400 mM diammonium phosphate, pH 7.4, and 10% (w/v) PEG 3350 |
