9UXV

Crystal structure of GSDME

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	78.450, 78.450, 353.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.990 - 2.950
R-factor	0.2379
Rwork	0.236
R-free	0.27430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.766
Data reduction software	XDS (Version January 31, 2020)
Data scaling software	XSCALE (Version January 31, 2020)
Phasing software	PHASER (Version January 31, 2020)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.140	3.070
High resolution limit [Å]	2.950	2.950
Number of reflections	24418	2635
<I/σ(I)>	1.47
Completeness [%]	99.9
Redundancy	14.2
CC(1/2)	0.999	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	20% (w/v) PEG3350, 8% (v/v) TacsimateTM pH 8.0