9UWZ

Crystal structure of the type III secretion chaperone VecA from Vibrio parahaemolyticus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.25380
Spacegroup name	P 41 21 2
Unit cell lengths	73.754, 73.754, 109.906
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.810 - 2.200
R-factor	0.2448
Rwork	0.239
R-free	0.29490
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.120	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.120	1.612
Rmeas	0.122	1.646
Rpim	0.025	0.329
Number of reflections	16059	1354
<I/σ(I)>	20.9
Completeness [%]	100.0
Redundancy	23.8	24.6
CC(1/2)	0.999	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M Tris-HCl (pH 8.8), 1.8M ammonium sulfate