9UUK

Crystal structure of the mu2 subunit of the clathrin-adaptor protein 2 (AP2) bound to HPV16 E7(residues 22-32; S31E and S32E)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2024-06-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 64
Unit cell lengths	125.206, 125.206, 73.492
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.794 - 3.199
R-factor	0.2092
Rwork	0.205
R-free	0.24380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.213
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.199	3.199
Rmerge	0.063	0.367
Number of reflections	10719	522
<I/σ(I)>	17.5
Completeness [%]	97.4
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.5 M sodium chloride and 100 mM potassium phosphate monobasic/sodium phosphate dibasic (pH 6.2)