9UU2

Outer membrane protein OmpA-c-like domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-01-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 31 2 1
Unit cell lengths	44.369, 44.369, 102.746
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	22.180 - 1.900
R-factor	0.2167
Rwork	0.213
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.734
Data reduction software	HKL-3000
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.810	1.968
High resolution limit [Å]	1.900	1.900
Rmeas	0.127	1.248
Number of reflections	9756	9756
<I/σ(I)>	1.16	1.16
Completeness [%]	99.6
Redundancy	16.6
CC(1/2)		0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	1% Tryptone, 0.001M sodium azide, 0.05M HEPES sodium pH 7.0, 20% w/v PEG3350.