9UIY

PLASMODIUM VIVAX PHENYLALANYL-TRNA SYNTHETASE IN COMPLEX WITH L-PHE IN SPACE GROUP P43212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-25
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	206.909, 206.909, 73.519
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.153 - 3.251
R-factor	0.2174
Rwork	0.214
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7by6
RMSD bond length	0.011
RMSD bond angle	1.339
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.15rc1_3423: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	146.310	3.310
High resolution limit [Å]	3.250	3.250
Rmeas	0.110	2.478
Number of reflections	25727	1431
<I/σ(I)>	5.6	0.5
Completeness [%]	100.0	100
Redundancy	27	26.9
CC(1/2)	1.000	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	12%W/V PEG 3350 and 0.1 M Ammonium tartarate dibasic pH 7.0 (PEG-ION G5)