9UIW

Plasmodium vivax cytoplasmic Phenylalanyl-tRNA synthetase in complex with Phenylalanine in space group P21212 at 2.60 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 2
Unit cell lengths	73.720, 136.170, 120.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.730 - 2.600
R-factor	0.2145
Rwork	0.213
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7by6
RMSD bond length	0.009
RMSD bond angle	1.016
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.730	2.760
High resolution limit [Å]	2.600	2.600
Rmerge	0.161	3.131
Rmeas	0.167	3.250
Number of reflections	38197	6022
<I/σ(I)>	12.1	0.6
Completeness [%]	99.8	98.9
Redundancy	13.7
CC(1/2)	0.999	0.440

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M magnesium acetate, 0.1 M MOPS and 12% w/v PEG 8000 (Proplex E8)