9UDY

Crystal structure of recombinant mushroom Agaricus bisporus mannose-binding protein with a longer C-terminal region

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-08
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	40.164, 43.982, 67.247
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.810 - 1.510
R-factor	0.13511
Rwork	0.132
R-free	0.19376
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eha
RMSD bond length	0.007
RMSD bond angle	1.365
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.980	1.540
High resolution limit [Å]	1.510	1.510
Rmerge	0.048	0.649
Rmeas	0.052	0.743
Rpim	0.021	0.356
Total number of observations	119287	3940
Number of reflections	19365	946
<I/σ(I)>	21.3	2
Completeness [%]	100.0
Redundancy	6.2	4.2
CC(1/2)	1.000	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	sodium formate